生物学原子力显微镜探针

Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers

Supplementary Figure 10 from Claire Leclech et al. (2023) “Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers”: Characterization of the secreted basement membrane. (C) Surface topography and stiffness (Young’s modulus) obtained by atomic force microscopy of the secreted ECM or bare PDMS after 72 h of culture on flat surfaces coated with fibronectin (FN) or poly-L-lysine (PLL). Dots represent individual measures on n=2 independent experiments. Error bars represent standard deviations. Please follow the link to the full article to find the full supplementary figure 10 and its description. Atomic Force Microscopy measurements were performed using an AFM probe with circular symmetric rounded AFM tip with typical radius of curvature around 30 nm (0.03–0.09 N m−1) (uniqprobe qp-BIOAC-CI, from NANOSENSORS).

In many tissues, cell shape and orientation are controlled by a combination of internal and external biophysical cues. * Cell shape is closely controlled in… Read More »Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers

Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation

Figure 5 from Catharina Husteden et al. 2023 “Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation”: A–D) Topography images [Cs/Col]4Cs (A), [Cs/Col]4Cs/LPX (B), [Cs/Col]4Cs/LPX/Cs (C), and [Cs/Col]4Cs/LPX/Cs/Col determined by AFM [Scale bar 1 µm] (D). E) Distribution curves of Young's modulus (E0) with a force map of a defined area (see also Figure S4, Supporting Information of the cited article). NANOSENSORSTM uniqprobe triangular qp-BioT AFM probes were used for the quantitative imaging to investigate the surface roughness and topography (in a standard liquid cell of a commercially available atomic force microscope) and force mapping. The uniqprobe qp-BioT AFM probe types offer an alternative to silicon nitride probes, with the advantage of taller AFM tips with smaller opening angles and reduced thermal drift.

The development of smart multifunctional biomaterials with the ability to control the behavior of stem cells on demand has become a powerful strategy in regenerative… Read More »Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation

Mitochondrial-derived vesicles retain membrane potential and contain a functional ATP synthase

by NANOSENSORS
15 March 202415 March 2024
Science & Technology

Figure 2 B from Reut Hazan et al. 2023 “Mitochondrial‐derived vesicles retain membrane potential and contain a functional ATP synthase”: Characterization of MDVs from wild‐type mitochondria B. Atomic force microscopy. Representative AFM image and a 3D AFM image of one representative vesicle (WT), adsorbed on a mica modified with Mg2+ and imaged under PBS. Please refer to the full article cited below to view the full figure. NANOSENSORSTM uniqprobeTM qp-BioAC-CI AFM probes with rounded AFM tips designed for cell imaging were used for the characterization by atomic force microscopy (AFM) in QI mode.

Vesicular transport is a means of communication. * While cells can communicate with each other via secretion of extracellular vesicles, less is known regarding organelle‐to… Read More »Mitochondrial-derived vesicles retain membrane potential and contain a functional ATP synthase

Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface

Figure 5 from “Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface” by Hassiba Smida et al. AFM images recorded in water of (A and C) CF and (B and D) Pt UME surfaces (A and B) before and (C and D) after a chronoamperometry measurement at +0.8 V vs Ag/AgCl in 2 mL of 20 mM K4Fe(CN)6 0.1 M PBS at pH 7.4 as aqueous solution under inert atmosphere (N2) in the presence of 109 cells of living SOMR1 bacteria sample. The circle in white dashed line is added for showing the UME disk on the AFM images. NANOSENSORS uniqprobe qp-SCONT AFM probes were used.

In the past few years, several novel analytical methods with high sensitivity and spatial-temporal resolution that enable qualitative and quantitative analysis at single-cell and subcellular… Read More »Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface

Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila

by NANOSENSORS
28 October 202215 March 2023
Science & Technology

Fig. 5 from “Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila” by Javier Arturo Sanchez-Lopez et al.: The spermatheca releases EVs with specific characteristics. a–e The spermathecae (SSC > CD63-GFP) were cultured ex vivo to characterize the particles that were released to the spent media: a size distribution of CD63-GFP-positive particles (n = 25 spermathecae from 15 flies) using single-particle tracking of the Nanoimager. b Characterization of CD63-GFP particles by ExoView (100 spermathecae from 50-60 flies) using anti-GFP or anti-CD63. c Representative dSTORM image of EVs found in the spent media of SSC expressing CD63-GFP (n = 25 spermathecae from 15 flies), stained with AlexaFluor555 conjugated anti-CD63 primary antibody. The image shows CD63-GFP-positive EVs and single CD63 AlexaFluor555 molecules the EV’s surface. Scale bars = 1 µm and insets = 20 nm. d–e The morphology of EVs in the spent media was observed by e negative staining in STEM; scale bar = 200 nm; and by d Cryo-TEM; Scale bar = 100 nm (see also Supplementary Fig. 5a-c). f–i The spent media of ex vivo cultured spermathecae were analyzed for the presence of EVs: f Representative AFM scans of SSC-EVs isolated by acoustic sorting (n = 100 spermathecae from 70 flies), showing from left to right: height (nm), adhesion (pN) and Young’s modulus images (MPa). Scale bars = 100 nm (see Supplementary Fig. 5d–g). g–i Single-particle tracking in the nanoimager. g CellMaskTM-positive particle size distribution and concentration profiles of EVs in the spent media of spermathecae incubated in media alone (No ecdy) or with ecdysone (Ecdy) (see also Supplementary Movie 5 for a time lapse of spermathecae end apparatus and endosomal activity post-ecdysone stimulation and methods, ex vivo spermatheca culture section); media with only CellMaskTM stain served as a control for the formation of dye aggregates. h Particle concentration and i mean particle diameter (nm) from g; Box plots are the measurements of particles from four frames of spent media from 25 spermathecae; boxes represent maximum, median and minimum values with outliers; one-way ANOVA, with nonparametric Wilcoxon multiple comparison post-hoc test; *p

The female reproductive tract (female-RT) must decipher the repertoire of molecular cues received from the male during copulation in order to activate and coordinate tract… Read More »Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila