AFM Tips

Comparative analysis of frictional behavior and mechanism of molybdenum ditelluride with different structures

by NANOSENSORS
11 September 2023
Science & Technology

Two-dimensional (2D) transition metal dichalcogenides (TMDCs) have layered structures with excellent tribological properties. * Since the energy difference between hexagonal-molybdenum ditelluride (2H-MoTe2) and distorted octahedral-molybdenum… Read More »Comparative analysis of frictional behavior and mechanism of molybdenum ditelluride with different structures

Strong, tough, rapid-recovery, and fatigue-resistant hydrogels made of picot peptide fibres

Fig. 2 from Bin Xue et al. “Strong, tough, rapid-recovery, and fatigue-resistant hydrogels made of picot peptide fibres”: Characterisation of supramolecular and picot GK11 peptide fibres without and with metal Cu2+ coordination. a AFM images of the fibres formed by the self-assembly of GK11 in the absence (top) and presence (bottom) of Cu2+. The graphs below the images correspond to the diameter distributions of the fibres. Scale bar = 1 μm. b, c CD spectrum (b) and FT-IR spectroscopy (c) of the self-assembled GK11 peptide with (GK11/Cu2+) and without Cu2+ (GK11). d As for (a), but for picot fibres (top) and metal ion-clad picot fibres (bottom) formed by GK11 linked with polyacrylamide in the absence and presence of Cu2+. Scale bar = 1 μm. NANOSENSORS PointProbe Plus PPP-SEIHR AFM probes for soft tapping mode were used.

Hydrogels are promising soft materials as tissue engineering scaffolds, stretchable sensors, and soft robotics. * Yet, it remains challenging to develop synthetic hydrogels with mechanical… Read More »Strong, tough, rapid-recovery, and fatigue-resistant hydrogels made of picot peptide fibres

Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface

Figure 5 from “Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface” by Hassiba Smida et al. AFM images recorded in water of (A and C) CF and (B and D) Pt UME surfaces (A and B) before and (C and D) after a chronoamperometry measurement at +0.8 V vs Ag/AgCl in 2 mL of 20 mM K4Fe(CN)6 0.1 M PBS at pH 7.4 as aqueous solution under inert atmosphere (N2) in the presence of 109 cells of living SOMR1 bacteria sample. The circle in white dashed line is added for showing the UME disk on the AFM images. NANOSENSORS uniqprobe qp-SCONT AFM probes were used.

In the past few years, several novel analytical methods with high sensitivity and spatial-temporal resolution that enable qualitative and quantitative analysis at single-cell and subcellular… Read More »Single Electrochemical Impacts of Shewanella oneidensis MR-1 Bacteria for Living Cells Adsorption onto a Polarized Ultramicroelectrode Surface

Happy New Lunar Year of the Rabbit!

by NANOSENSORS
21 January 202315 March 2023
News & Events

Drawing of two rabbits in front of the full moon with happy new lunar year written in the grass surrounding them.

Season’s Greetings from NANOSENSORS AFM probes

by NANOSENSORS
22 December 202215 March 2023
News & Events

Illuminated Christmas tree at night time beside an old building

It is again time to wish all users of our AFM probes Happy Holidays! Enjoy the holiday season with friends and family. We are looking… Read More »Season’s Greetings from NANOSENSORS AFM probes

Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila

by NANOSENSORS
28 October 202215 March 2023
Science & Technology

Fig. 5 from “Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila” by Javier Arturo Sanchez-Lopez et al.: The spermatheca releases EVs with specific characteristics. a–e The spermathecae (SSC > CD63-GFP) were cultured ex vivo to characterize the particles that were released to the spent media: a size distribution of CD63-GFP-positive particles (n = 25 spermathecae from 15 flies) using single-particle tracking of the Nanoimager. b Characterization of CD63-GFP particles by ExoView (100 spermathecae from 50-60 flies) using anti-GFP or anti-CD63. c Representative dSTORM image of EVs found in the spent media of SSC expressing CD63-GFP (n = 25 spermathecae from 15 flies), stained with AlexaFluor555 conjugated anti-CD63 primary antibody. The image shows CD63-GFP-positive EVs and single CD63 AlexaFluor555 molecules the EV’s surface. Scale bars = 1 µm and insets = 20 nm. d–e The morphology of EVs in the spent media was observed by e negative staining in STEM; scale bar = 200 nm; and by d Cryo-TEM; Scale bar = 100 nm (see also Supplementary Fig. 5a-c). f–i The spent media of ex vivo cultured spermathecae were analyzed for the presence of EVs: f Representative AFM scans of SSC-EVs isolated by acoustic sorting (n = 100 spermathecae from 70 flies), showing from left to right: height (nm), adhesion (pN) and Young’s modulus images (MPa). Scale bars = 100 nm (see Supplementary Fig. 5d–g). g–i Single-particle tracking in the nanoimager. g CellMaskTM-positive particle size distribution and concentration profiles of EVs in the spent media of spermathecae incubated in media alone (No ecdy) or with ecdysone (Ecdy) (see also Supplementary Movie 5 for a time lapse of spermathecae end apparatus and endosomal activity post-ecdysone stimulation and methods, ex vivo spermatheca culture section); media with only CellMaskTM stain served as a control for the formation of dye aggregates. h Particle concentration and i mean particle diameter (nm) from g; Box plots are the measurements of particles from four frames of spent media from 25 spermathecae; boxes represent maximum, median and minimum values with outliers; one-way ANOVA, with nonparametric Wilcoxon multiple comparison post-hoc test; *p

The female reproductive tract (female-RT) must decipher the repertoire of molecular cues received from the male during copulation in order to activate and coordinate tract… Read More »Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila

First day @Arablab

by NANOSENSORS
24 October 202215 March 2023
News & Events

Meet our CEO at Arablab 2022 and discuss the many applications that are possible using our AFM probes for Atomic Force Microscopy

Meet our CEO @Arablab 2022 in Dubai this week and discuss the many applications that are possible using our #AFMprobes for #AtomicForceMicroscopy