AFM Tips

Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila

by NANOSENSORS
28 October 202215 March 2023
Science & Technology

Fig. 5 from “Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila” by Javier Arturo Sanchez-Lopez et al.: The spermatheca releases EVs with specific characteristics. a–e The spermathecae (SSC > CD63-GFP) were cultured ex vivo to characterize the particles that were released to the spent media: a size distribution of CD63-GFP-positive particles (n = 25 spermathecae from 15 flies) using single-particle tracking of the Nanoimager. b Characterization of CD63-GFP particles by ExoView (100 spermathecae from 50-60 flies) using anti-GFP or anti-CD63. c Representative dSTORM image of EVs found in the spent media of SSC expressing CD63-GFP (n = 25 spermathecae from 15 flies), stained with AlexaFluor555 conjugated anti-CD63 primary antibody. The image shows CD63-GFP-positive EVs and single CD63 AlexaFluor555 molecules the EV’s surface. Scale bars = 1 µm and insets = 20 nm. d–e The morphology of EVs in the spent media was observed by e negative staining in STEM; scale bar = 200 nm; and by d Cryo-TEM; Scale bar = 100 nm (see also Supplementary Fig. 5a-c). f–i The spent media of ex vivo cultured spermathecae were analyzed for the presence of EVs: f Representative AFM scans of SSC-EVs isolated by acoustic sorting (n = 100 spermathecae from 70 flies), showing from left to right: height (nm), adhesion (pN) and Young’s modulus images (MPa). Scale bars = 100 nm (see Supplementary Fig. 5d–g). g–i Single-particle tracking in the nanoimager. g CellMaskTM-positive particle size distribution and concentration profiles of EVs in the spent media of spermathecae incubated in media alone (No ecdy) or with ecdysone (Ecdy) (see also Supplementary Movie 5 for a time lapse of spermathecae end apparatus and endosomal activity post-ecdysone stimulation and methods, ex vivo spermatheca culture section); media with only CellMaskTM stain served as a control for the formation of dye aggregates. h Particle concentration and i mean particle diameter (nm) from g; Box plots are the measurements of particles from four frames of spent media from 25 spermathecae; boxes represent maximum, median and minimum values with outliers; one-way ANOVA, with nonparametric Wilcoxon multiple comparison post-hoc test; *p

The female reproductive tract (female-RT) must decipher the repertoire of molecular cues received from the male during copulation in order to activate and coordinate tract… Read More »Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila

First day @Arablab

by NANOSENSORS
24 October 202215 March 2023
News & Events

Meet our CEO at Arablab 2022 and discuss the many applications that are possible using our AFM probes for Atomic Force Microscopy

Meet our CEO @Arablab 2022 in Dubai this week and discuss the many applications that are possible using our #AFMprobes for #AtomicForceMicroscopy

Toward Single-Atomic-Layer Lithography on Highly Oriented Pyrolytic Graphite Surfaces Using AFM-Based Electrochemical Etching

by NANOSENSORS
9 October 202215 March 2023
Science & Technology

Figure 2 from Wei Han et al. “Toward Single-Atomic-Layer Lithography on Highly Oriented Pyrolytic Graphite Surfaces Using AFM-Based Electrochemical Etching” Schematic diagram of the AFM-based electrochemical etching apparatus with an RH-controlled environment. The experiments were performed under ambient conditions with a commercial atomic force microscope using NANOSENSORS PtIr5 coated PointProbePlus® PPP-EFM AFM probes. The AFM tip side coating enhances the conductivity of the AFM tip and allows electrical contacts, and the opposite side coating enhances the laser reflex.

Happy #NanotechnologyDay! The date, 10/9, is a nod to the nanometer scale, where objects span only billionths of a meter (10⁻⁹ meters). We celebrate it… Read More »Toward Single-Atomic-Layer Lithography on Highly Oriented Pyrolytic Graphite Surfaces Using AFM-Based Electrochemical Etching

Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments

by NANOSENSORS
14 September 202215 March 2023
Science & Technology, Uncategorized

Figure 3 from “Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments” by Miguel Cantero et al.: Treatment of TGEV with IGEPAL 0.2% (A). Topographical images before (left) and after (right) IGEPAL treatment (B). Profiles traced over the particles before (black) and after (blue) the treatment. The time interval between images was ~30 s (C). Height distribution of TGEV particles before (black) and after (blue) treatment (n = 103). Counts taken from the distribution curve were normalized for comparison. The peak shifts from the value of the intact particle height to the height of the cores. NANOSENSORS uniqprobe qp-BioAC AFM probes were used for the atomic force microscopy measurements.

Successful airborne transmission of coronaviruses through fluid microdroplets requires a virion structure that must withstand harsh natural conditions. * Because of the strict biosafety requirements… Read More »Monitoring SARS-CoV-2 Surrogate TGEV Individual Virions Structure Survival under Harsh Physicochemical Environments

Elastic shell theory for plant cell wall stiffness reveals contributions of cell wall elasticity and turgor pressure in AFM measurement

by NANOSENSORS
4 August 202215 March 2023
Science & Technology

Fig-3-S-Tsugawa-et-al-2022-Elastic-shell-theory-for-plant-cell-wall-stiffness-reveals-contributions-of-cell-wall-elasticity-and-turgor-pressure-in-AFM-measurement NANOSENSORS Sphere-AFM probes SD-Sphere-NCH-S from the NANOSENSORS™ Special Developments List were used for the force-indentation curve measurements with atomic force microscopy

The stiffness of a plant cell in response to an applied force is determined not only by the elasticity of the cell wall but also… Read More »Elastic shell theory for plant cell wall stiffness reveals contributions of cell wall elasticity and turgor pressure in AFM measurement

Happy Swiss National Holiday with Smallest Swiss Cross

by NANOSENSORS
1 August 202215 March 2023
News & Events

We are celebrating today’s Swiss National Holiday courtesy of Basel University with the Smallest Swiss Cross – Made of 20 Single Atoms ( a NANOSENSORS… Read More »Happy Swiss National Holiday with Smallest Swiss Cross

Stress- and Time-Dependent Formation of Self-Lubricating In Situ Carbon (SLIC) Films on Catalytically-Active Noble Alloys

by NANOSENSORS
16 June 202215 March 2023
Science & Technology

Figure 5 from Morgan R. Jones et al. Stress- and Time-Dependent Formation of Self-Lubricating In Situ Carbon (SLIC) Films on Catalytically-Active Noble Alloys: Tribofilm formation as a function of number of cycles as determined from the nanoscale experiments. (a) Intermittent-contact mode topography images (3 µm × 3 µm area) of the contact region at a contact pressure of 1.2 GPa after 0 cycles, 500 cycles, 1000 cycles, 2000 cycles, 3000 cycles, and 4000 cycles. (b) Tribofilm volume as a function of cycles for contact pressures up to 3.1 GPa. For all P, tribofilm volume increased asymptotically to a steady-state value at large numbers of cycles. NANOSENSORS Diamond Coated PointProbe Plus AFM probes DT-CONTR were used for the nanoscale tribology with atomic force microscopy.

Although catalysis is a popular explanation for tribopolymer generation, the interplay of catalysis, mechanochemistry, and electrostatic interactions remain incompletely understood. There is consensus, however, that… Read More »Stress- and Time-Dependent Formation of Self-Lubricating In Situ Carbon (SLIC) Films on Catalytically-Active Noble Alloys