biophysics

Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers

Supplementary Figure 10 from Claire Leclech et al. (2023) “Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers”: Characterization of the secreted basement membrane. (C) Surface topography and stiffness (Young’s modulus) obtained by atomic force microscopy of the secreted ECM or bare PDMS after 72 h of culture on flat surfaces coated with fibronectin (FN) or poly-L-lysine (PLL). Dots represent individual measures on n=2 independent experiments. Error bars represent standard deviations. Please follow the link to the full article to find the full supplementary figure 10 and its description. Atomic Force Microscopy measurements were performed using an AFM probe with circular symmetric rounded AFM tip with typical radius of curvature around 30 nm (0.03–0.09 N m−1) (uniqprobe qp-BIOAC-CI, from NANOSENSORS).

In many tissues, cell shape and orientation are controlled by a combination of internal and external biophysical cues. * Cell shape is closely controlled in… Read More »Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers

Celebrating 10 years NANOSENSORS Uniqprobe uniform quality AFM probes

NANOSENSORS uniqprobe AFM cantilever with partial coating

10 years ago at MRS Fall 2013 NANOSENSORS introduced the first types of the Uniqprobe – uniform quality SPM probes series. During these 10 years… Read More »Celebrating 10 years NANOSENSORS Uniqprobe uniform quality AFM probes

Elastic shell theory for plant cell wall stiffness reveals contributions of cell wall elasticity and turgor pressure in AFM measurement

by NANOSENSORS
4 August 202215 March 2023
Science & Technology

Fig-3-S-Tsugawa-et-al-2022-Elastic-shell-theory-for-plant-cell-wall-stiffness-reveals-contributions-of-cell-wall-elasticity-and-turgor-pressure-in-AFM-measurement NANOSENSORS Sphere-AFM probes SD-Sphere-NCH-S from the NANOSENSORS™ Special Developments List were used for the force-indentation curve measurements with atomic force microscopy

The stiffness of a plant cell in response to an applied force is determined not only by the elasticity of the cell wall but also… Read More »Elastic shell theory for plant cell wall stiffness reveals contributions of cell wall elasticity and turgor pressure in AFM measurement

Cerosomes as skin repairing agent: Mode of action studies with a model stratum corneum layer at liquid/air and liquid/solid interfaces

by NANOSENSORS
23 March 202215 March 2023
Science & Technology

Fig. 7 from “Cerosomes as skin repairing agent: Mode of action studies with a model stratum corneum layer at liquid/air and liquid/solid interfaces” by F. Strati et al: AFM scans of a) SC model monolayer transferred via LB method onto mica support, b) SC model monolayer after injection of cerosomes, c) SC model monolayer after injection of cerosome + S75-3 formulation, and d) SC model monolayer after injection of S75-3 liposomal formulation. All samples were transferred via the LS method onto glass substrate. Each experiment was performed at 20°C and the subphase used for a) was Millipore water while for b), c), and d) the same aqueous solutions have been used as for the formulation of the liposomes. Topographical images were recorded in liquid state using NANOSENSORS uniqprobe qp-BioT AFM probes in a standard liquid cell containing the needed buffer.

The human skin is the second largest organ of the human body. The stratum corneum (SC) is the outmost layer of the human skin and… Read More »Cerosomes as skin repairing agent: Mode of action studies with a model stratum corneum layer at liquid/air and liquid/solid interfaces

Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

by NANOSENSORS
9 June 202015 March 2023
Science & Technology

Figure 4 a and b from Ana I. Gómez-Varela et al “Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences : Simultaneous SR-SIM/AFM acquisition. The AFM measurements were carried out on fixed U2OS cells in medium/buffer with (a) and without N-SIM illumination (b). For convenience and enhanced feature/noise contrast, both AFM topography images in the SR-SIM/AFM overlays are displayed with an edge detection algorithm using a pixel difference operator in X. The topography images from Petri dish surface on three positions (labelled in the figures) were planefit (1st order polynomial function) to compensate for tilts in the sample surface, and subjected to surface roughness analysis Please have a look at the full article to view the full figure. https://rdcu.be/b4Iot

Correlating data from different microscopy techniques holds the potential to discover new facets of signalling events in cellular biology.* In the article “Simultaneous co-localized super-resolution… Read More »Simultaneous co-localized super-resolution fluorescence microscopy and atomic force microscopy: combined SIM and AFM platform for the life sciences

Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. Elegans

by NANOSENSORS
14 April 202015 March 2023
Science & Technology

Genetic and environmental factors are key drivers regulating organismal lifespan but how these impact healthspan is less well understood. Techniques capturing biomechanical properties of tissues… Read More »Mechanical properties measured by atomic force microscopy define health biomarkers in ageing C. Elegans

Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

by NANOSENSORS
23 September 201915 March 2023
Science & Technology

The mechanical properties and structural organization of membranes determine the functional state of red blood cells (RBCs). Deformability is one of the key physiological and… Read More »Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action