contact mode

Quantifying mechanical forces during vertebrate morphogenesis

Fig. 1 from : Eirini Maniou et al. 2024 “Quantifying mechanical forces during vertebrate morphogenesis”: The i3D bioprinting with accurately determined position, geometry and stiffness. a, Schematic of a chicken embryo illustrating the experimental workflow: 2–3 µl i3D polymer is pipetted directly onto the rhombocervical neuropore (RNP) and photo-crosslinked with a two-photon laser. The iMeSH structures are shown in green throughout. b, Stereoscope image of an embryo with a star shape photo- crosslinked on the flat neural plate. Scale bars, 200 µm. The star dimensions are indicated in the inset. c, Schematic showing iMeSH compression by apposition of the neural folds. d, Time-lapse images showing the sequential displacement of a rigid iMeSH shape, shown as a 3D confocal reconstruction superimposed on the embryo imaged with transmitted light. Cyan shading, open neural tube; *, zippering point; arrow indicates rotation of the printed shape; scale bar, 50 µm. The times are shown. e, Fire lookup table showing the autofluorescence of iMeSH photo-crosslinked with the indicated laser powers on the same embryo. Scale bar, 25 µm. f, Schematic illustration of AFM stiffness testing of an iMeSH shape; 3D reconstructions of the shape are shown superimposed on a dorsal and transverse schematic of the embryo. Scale bar, 100 µm. g, AFM quantification of iMeSH crosslinked on an embryo at the indicated laser powers. The values were calculated from AFM indentations performed at a rate of 0.5 μm s–1 and depths of 1 μm (30% 7-hydroxycoumarin-3-carboxylic acid (HCC) polyethylene glycol (PEG)) or 2 μm (15% PEG). The force–displacement curves were acquired using NANOSENSORS™ PointProbe® Plus PPP-CONTSCR silicon AFM probes with a typical spring constant of 0.2 N/m.

Morphogenesis requires embryonic cells to generate forces and perform mechanical work to shape their tissues. Incorrect functioning of these force fields can lead to congenital… Read More »Quantifying mechanical forces during vertebrate morphogenesis

Celebrating 10 years NANOSENSORS Uniqprobe uniform quality AFM probes

NANOSENSORS uniqprobe AFM cantilever with partial coating

10 years ago at MRS Fall 2013 NANOSENSORS introduced the first types of the Uniqprobe – uniform quality SPM probes series. During these 10 years… Read More »Celebrating 10 years NANOSENSORS Uniqprobe uniform quality AFM probes

Kelvin probe force microscopy work function characterization of transition metal oxide crystals under ongoing reduction and oxidation

by NANOSENSORS
19 January 202015 March 2023
Science & Technology

Controlling the work function of transition metal oxides is of key importance with regard to future energy production and storage. As the majority of applications… Read More »Kelvin probe force microscopy work function characterization of transition metal oxide crystals under ongoing reduction and oxidation

Single layer graphene induces load-bearing molecular layering at the hexadecane-steel interface

by NANOSENSORS
11 December 201915 March 2023
Science & Technology

Carbon-based layers play an important role in boundary lubrication, from graphite as commercial solid lubricant in a spray can over diamond-like carbon coatings in automotive… Read More »Single layer graphene induces load-bearing molecular layering at the hexadecane-steel interface

Nucleation in confinement generates long-range repulsion between rough calcite surfaces

by NANOSENSORS
5 August 201915 March 2023
Science & Technology

Fluid-induced alteration of rocks and mineral-based materials often starts at confined mineral interfaces where nm-thick water films can persist even at high overburden pressures and… Read More »Nucleation in confinement generates long-range repulsion between rough calcite surfaces

Substrate properties modulate cell membrane roughness by way of actin filaments

by NANOSENSORS
4 January 201915 March 2023
Science & Technology

Supplementary Figure S1 from Chao-Hung Chang et al. “Substrate properties modulate cell membrane roughness by way of actin filaments”: Images of membrane topography determined by atomic force microscopy (AFM). MEFs were seeded on the polymer coverslip-bottom μ-dishes coated with 0 or 10 μg/ml FN for 6 hours for the measurement of membrane roughness by AFM. The regions marked by the white squares in the bright-field images are displayed in the membrane topography. Scale bar, 10 μm. NANOSENSORS uniqprobe qp-SCONT AFM probes(long cantilever length 125 um, spring constant 0.01 N/m) were used.

“Cell membrane roughness has been proposed as a sensitive feature to reflect cellular physiological conditions”* In the article “Substrate properties modulate cell membrane roughness by… Read More »Substrate properties modulate cell membrane roughness by way of actin filaments

Consistent AFM tip shape leading to reproducible results – NANOSENSORS PointProbe Plus Screencast passes 500 views mark

by NANOSENSORS
28 April 201715 March 2023
Videos

The screencast held by Head of R&D Thomas Sulzbach on the NANOSENSORS PointProbe® Plus Silicon AFM probe series with a consistent tip shape leading to… Read More »Consistent AFM tip shape leading to reproducible results – NANOSENSORS PointProbe Plus Screencast passes 500 views mark