force measurements

Calibration Service for AFM Cantilevers

The NANOSENSORS screencast on the calibration service for AFM cantilevers has just reached the 1500 views mark. Accurately determined AFM cantilever properties are very important…

by NANOSENSORS
29 October 202120 November 2022
Science & Technology

Figure 3 from “Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds AFM imaging and spectrometry measurement” by Ashlee F. Harris et al.: They used AFM surface topography measurements to confirm that the structures such as plant vasculature were preserved after the scSO2 process and used AFM force curves to calculate the Young’s Modulus (YM) of the scCO2 decellularized scaffold. NANOSENSORS uniqprobe qp-BioAC AFM probes were used for the described AFM measurments. (a) Representative false colored three-dimensional surface mapping images and (b) Young’s modulus of scCO2 and chemically decellularized scaffolds (data as mean ± SEM; n = 5).

Biocompatible scaffolds that can be repopulated with human cells have many uses such serving as replacement organs and tissues. Therefore there is an increasing interest…

by NANOSENSORS
3 February 202120 November 2022
Science & Technology

Figure 4 from “Cytoskeletal disorganization underlies PABPN1-mediated myogenic disability” by Cyriel Sebastiaan Olie et al. Disrupted cytoskeletal spatial organization in shPAB human muscle cell cultures. A Representative images of control and shPAB human muscle cell cultures, stained with antibodies to tubulin and actin, and the actin filaments were visualized with actin-GFP. B Tubulin staining in control and shPAB myoblast cell cultures after DMSO, 100 nM nocodazole or 25 nM paclitaxel treatment for 2 h. Scale bar is 25 µm. C Measurements of cell-mechanics properties in control and shPAB cells using the Brillouin Light Scattering Microscopy (Ci) or the Atomic Force Microscopy (Cii). Measurements were carried out in myoblasts; every dot represents the median from 1000 measurements in a cell. Cell stiffness is measured by GHz, and the young modulus reports the cell surface tension. Averages and standard deviations are from N = 15 cells. Statistical significance was calculated with the student’s t-test. NANOSENSORS uniqprobe qp-BioAC ( CB3 ) AFM probes were used in the quantitative imaging of cell-mechanics properties where a force curve is applied at each point.

Muscle wasting is connected with changes in various cellular mechanisms that influence protein homeostasis, transcription, protein acetylation and different metabolic pathways. * Scientific studies have…

by NANOSENSORS
7 September 202020 November 2022
Science & Technology

Plant-based scaffolds present many advantages over a variety of biomaterials.* Recent studies explored their potential to be repopulated with human cells and thus highlight a…

by NANOSENSORS
11 December 201920 November 2022
Science & Technology

Carbon-based layers play an important role in boundary lubrication, from graphite as commercial solid lubricant in a spray can over diamond-like carbon coatings in automotive…

by NANOSENSORS
23 September 201920 November 2022
Science & Technology

The mechanical properties and structural organization of membranes determine the functional state of red blood cells (RBCs). Deformability is one of the key physiological and…

by NANOSENSORS
1 September 201920 November 2022
Science & Technology

Mechanical forces have emerged as coordinating signals for most cell functions. Yet, because forces are invisible, mapping tensile stress patterns in tissues remains a major…

force measurements

Calibration Service for AFM Cantilevers

Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds

Cytoskeletal disorganization underlies PABPN1-mediated myogenic disability

Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models

Single layer graphene induces load-bearing molecular layering at the hexadecane-steel interface

Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

A tension-adhesion feedback loop in plant epidermis