force spectroscopy

Quantifying mechanical forces during vertebrate morphogenesis

Fig. 1 from : Eirini Maniou et al. 2024 “Quantifying mechanical forces during vertebrate morphogenesis”: The i3D bioprinting with accurately determined position, geometry and stiffness. a, Schematic of a chicken embryo illustrating the experimental workflow: 2–3 µl i3D polymer is pipetted directly onto the rhombocervical neuropore (RNP) and photo-crosslinked with a two-photon laser. The iMeSH structures are shown in green throughout. b, Stereoscope image of an embryo with a star shape photo- crosslinked on the flat neural plate. Scale bars, 200 µm. The star dimensions are indicated in the inset. c, Schematic showing iMeSH compression by apposition of the neural folds. d, Time-lapse images showing the sequential displacement of a rigid iMeSH shape, shown as a 3D confocal reconstruction superimposed on the embryo imaged with transmitted light. Cyan shading, open neural tube; *, zippering point; arrow indicates rotation of the printed shape; scale bar, 50 µm. The times are shown. e, Fire lookup table showing the autofluorescence of iMeSH photo-crosslinked with the indicated laser powers on the same embryo. Scale bar, 25 µm. f, Schematic illustration of AFM stiffness testing of an iMeSH shape; 3D reconstructions of the shape are shown superimposed on a dorsal and transverse schematic of the embryo. Scale bar, 100 µm. g, AFM quantification of iMeSH crosslinked on an embryo at the indicated laser powers. The values were calculated from AFM indentations performed at a rate of 0.5 μm s–1 and depths of 1 μm (30% 7-hydroxycoumarin-3-carboxylic acid (HCC) polyethylene glycol (PEG)) or 2 μm (15% PEG). The force–displacement curves were acquired using NANOSENSORS™ PointProbe® Plus PPP-CONTSCR silicon AFM probes with a typical spring constant of 0.2 N/m.

Morphogenesis requires embryonic cells to generate forces and perform mechanical work to shape their tissues. Incorrect functioning of these force fields can lead to congenital… Read More »Quantifying mechanical forces during vertebrate morphogenesis

Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models

by NANOSENSORS
7 September 202015 March 2023
Science & Technology

Plant-based scaffolds present many advantages over a variety of biomaterials.* Recent studies explored their potential to be repopulated with human cells and thus highlight a… Read More »Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models

Single layer graphene induces load-bearing molecular layering at the hexadecane-steel interface

by NANOSENSORS
11 December 201915 March 2023
Science & Technology

Carbon-based layers play an important role in boundary lubrication, from graphite as commercial solid lubricant in a spray can over diamond-like carbon coatings in automotive… Read More »Single layer graphene induces load-bearing molecular layering at the hexadecane-steel interface

Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

by NANOSENSORS
23 September 201915 March 2023
Science & Technology

The mechanical properties and structural organization of membranes determine the functional state of red blood cells (RBCs). Deformability is one of the key physiological and… Read More »Nonlinear Biomechanical Characteristics of Deep Deformation of Native RBC Membranes in Normal State and under Modifier Action

Rounded AFM Tips for Nanomechanics and Force Spectroscopy – Special Development List updated

by NANOSENSORS
20 April 201515 March 2023
News & Events, Science & Technology

Rounded AFM tip SD-R30 for nanomechanics

NANOSENSORS has added a new type of rounded AFM tips to the Special Development List. The new rounded AFM tips SD-R30 have a typical tip… Read More »Rounded AFM Tips for Nanomechanics and Force Spectroscopy – Special Development List updated