Scanning Probe Microscopy

Happy Holidays 2024 from NANOSENSORS AFM probes

by NANOSENSORS
20 December 202420 December 2024
News & Events

It is again time to wish all users of our #AFMprobes Happy Holidays! After we shared a “Halloween tree” in October we now finally have… Read More »Happy Holidays 2024 from NANOSENSORS AFM probes

Carbon Thin-Film Electrodes as High-Performing Substrates for Correlative Single Entity Electrochemistry

by NANOSENSORS
26 September 2024
Science & Technology

S15 from Marc Brunet Cabré et al 2024 “Carbon Thin-Film Electrodes as High-Performing Substrates for Correlative Single Entity Electrochemistry” SI-5: Morphology of isolated MXene (Ti3C2Tx) on anC by AFM Figure S15: AFM images of two different regions of anC substrate with dropcasted MXene flakes. (A) Displays an isolated flake on anC and (B) displays multiple flakes which present partial stacking between them. NANOSENSORS PointProbe® Plus PPP-NCHR AFM probes were used for the scanning probe microscopy (SPM) characterization in non-contact mode.

Single-entity electrochemistry (SEE) is an emerging area of research that aims at evaluating the electrochemical response of materials at the micro- and nanoscale. * Several… Read More »Carbon Thin-Film Electrodes as High-Performing Substrates for Correlative Single Entity Electrochemistry

Quantifying mechanical forces during vertebrate morphogenesis

Fig. 1 from : Eirini Maniou et al. 2024 “Quantifying mechanical forces during vertebrate morphogenesis”: The i3D bioprinting with accurately determined position, geometry and stiffness. a, Schematic of a chicken embryo illustrating the experimental workflow: 2–3 µl i3D polymer is pipetted directly onto the rhombocervical neuropore (RNP) and photo-crosslinked with a two-photon laser. The iMeSH structures are shown in green throughout. b, Stereoscope image of an embryo with a star shape photo- crosslinked on the flat neural plate. Scale bars, 200 µm. The star dimensions are indicated in the inset. c, Schematic showing iMeSH compression by apposition of the neural folds. d, Time-lapse images showing the sequential displacement of a rigid iMeSH shape, shown as a 3D confocal reconstruction superimposed on the embryo imaged with transmitted light. Cyan shading, open neural tube; *, zippering point; arrow indicates rotation of the printed shape; scale bar, 50 µm. The times are shown. e, Fire lookup table showing the autofluorescence of iMeSH photo-crosslinked with the indicated laser powers on the same embryo. Scale bar, 25 µm. f, Schematic illustration of AFM stiffness testing of an iMeSH shape; 3D reconstructions of the shape are shown superimposed on a dorsal and transverse schematic of the embryo. Scale bar, 100 µm. g, AFM quantification of iMeSH crosslinked on an embryo at the indicated laser powers. The values were calculated from AFM indentations performed at a rate of 0.5 μm s–1 and depths of 1 μm (30% 7-hydroxycoumarin-3-carboxylic acid (HCC) polyethylene glycol (PEG)) or 2 μm (15% PEG). The force–displacement curves were acquired using NANOSENSORS™ PointProbe® Plus PPP-CONTSCR silicon AFM probes with a typical spring constant of 0.2 N/m.

Morphogenesis requires embryonic cells to generate forces and perform mechanical work to shape their tissues. Incorrect functioning of these force fields can lead to congenital… Read More »Quantifying mechanical forces during vertebrate morphogenesis

Happy Swiss National Holiday 2024 with Smallest Swiss Cross

by NANOSENSORS
31 July 202431 July 2024
News & Events

20 bromine atoms positioned on a sodium chloride surface using the tip of an atomic force microscope at room temperature, creating a Swiss cross with the size of 5.6nm. The structure is stable at room temperature and was achieved by exchanging chlorine with bromine atoms. (Fig: University of Basel, Department of Physics) The image was taken using a NANOSENSORS PointProbePlus PPP-NCL AFM probe

We are celebrating tomorrow’s Swiss National Holiday courtesy of Basel University with the Smallest Swiss Cross – Made of 20 Single Atoms ( a NANOSENSORS… Read More »Happy Swiss National Holiday 2024 with Smallest Swiss Cross

Magnetic control of self-assembly and disassembly in organic materials

Figure 3 from You-jin Jung et al. 2023 “Magnetic control of self-assembly and disassembly in organic materials”: a MNP-helix rod-coils containing positively charged amino acid residues, Rx-α10-PEG16 (x = 1–4). The building blocks have two to five positive charges. b A linear plasmid DNA (7.5 kb). c Self-assembled nanostructures of R1-α10-PEG16. d EMSA on agarose gel. e The PD complexes (intermediates). Left: an AFM image, right: a TEM image. The charge ratio (+/−) was 0.7. f The PD complexes (nanoribbons). g The PD complexes (nanoribbons + artificial chromosomes). h The PD complexes (artificial chromosomes). i The molecular mechanism responsible for the formation of the nanoribbon. Atomic Force Microscopy was preformed in non-contact mode using NANOSENSORS PointProbe Plus PPP-NCHR AFM probes.

Magnetically responsive materials are highly valuable for biological and medical uses because the magnetic field is non-invasive, radiation-free, and safe for humans.* DNA and some… Read More »Magnetic control of self-assembly and disassembly in organic materials

Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers

Supplementary Figure 10 from Claire Leclech et al. (2023) “Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers”: Characterization of the secreted basement membrane. (C) Surface topography and stiffness (Young’s modulus) obtained by atomic force microscopy of the secreted ECM or bare PDMS after 72 h of culture on flat surfaces coated with fibronectin (FN) or poly-L-lysine (PLL). Dots represent individual measures on n=2 independent experiments. Error bars represent standard deviations. Please follow the link to the full article to find the full supplementary figure 10 and its description. Atomic Force Microscopy measurements were performed using an AFM probe with circular symmetric rounded AFM tip with typical radius of curvature around 30 nm (0.03–0.09 N m−1) (uniqprobe qp-BIOAC-CI, from NANOSENSORS).

In many tissues, cell shape and orientation are controlled by a combination of internal and external biophysical cues. * Cell shape is closely controlled in… Read More »Distinct Contact Guidance Mechanisms in Single Endothelial Cells and in Monolayers

Electron-beam lithography of nanostructures at the tips of scanning probe cantilevers

by NANOSENSORS
16 February 2024
Science & Technology

It is always exiting to see what our creative and innovative users are doing with our AFM probes. In their article “Electron-beam lithography of nanostructures… Read More »Electron-beam lithography of nanostructures at the tips of scanning probe cantilevers

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