Scanning Probe Microscopy

Season’s Greetings from NANOSENSORS AFM probes

by NANOSENSORS
22 December 2022
News & Events

Illuminated Christmas tree at night time beside an old building

It is again time to wish all users of our AFM probes Happy Holidays! Enjoy the holiday season with friends and family. We are looking…

by NANOSENSORS
28 October 202220 November 2022
Science & Technology

Fig. 5 from “Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila” by Javier Arturo Sanchez-Lopez et al.: The spermatheca releases EVs with specific characteristics. a–e The spermathecae (SSC > CD63-GFP) were cultured ex vivo to characterize the particles that were released to the spent media: a size distribution of CD63-GFP-positive particles (n = 25 spermathecae from 15 flies) using single-particle tracking of the Nanoimager. b Characterization of CD63-GFP particles by ExoView (100 spermathecae from 50-60 flies) using anti-GFP or anti-CD63. c Representative dSTORM image of EVs found in the spent media of SSC expressing CD63-GFP (n = 25 spermathecae from 15 flies), stained with AlexaFluor555 conjugated anti-CD63 primary antibody. The image shows CD63-GFP-positive EVs and single CD63 AlexaFluor555 molecules the EV’s surface. Scale bars = 1 µm and insets = 20 nm. d–e The morphology of EVs in the spent media was observed by e negative staining in STEM; scale bar = 200 nm; and by d Cryo-TEM; Scale bar = 100 nm (see also Supplementary Fig. 5a-c). f–i The spent media of ex vivo cultured spermathecae were analyzed for the presence of EVs: f Representative AFM scans of SSC-EVs isolated by acoustic sorting (n = 100 spermathecae from 70 flies), showing from left to right: height (nm), adhesion (pN) and Young’s modulus images (MPa). Scale bars = 100 nm (see Supplementary Fig. 5d–g). g–i Single-particle tracking in the nanoimager. g CellMaskTM-positive particle size distribution and concentration profiles of EVs in the spent media of spermathecae incubated in media alone (No ecdy) or with ecdysone (Ecdy) (see also Supplementary Movie 5 for a time lapse of spermathecae end apparatus and endosomal activity post-ecdysone stimulation and methods, ex vivo spermatheca culture section); media with only CellMaskTM stain served as a control for the formation of dye aggregates. h Particle concentration and i mean particle diameter (nm) from g; Box plots are the measurements of particles from four frames of spent media from 25 spermathecae; boxes represent maximum, median and minimum values with outliers; one-way ANOVA, with nonparametric Wilcoxon multiple comparison post-hoc test; *p

The female reproductive tract (female-RT) must decipher the repertoire of molecular cues received from the male during copulation in order to activate and coordinate tract…

by NANOSENSORS
24 October 202220 November 2022
News & Events

Meet our CEO at Arablab 2022 and discuss the many applications that are possible using our AFM probes for Atomic Force Microscopy

Meet our CEO @Arablab 2022 in Dubai this week and discuss the many applications that are possible using our #AFMprobes for #AtomicForceMicroscopy

Toward Single-Atomic-Layer Lithography on Highly Oriented Pyrolytic Graphite Surfaces Using AFM-Based Electrochemical Etching

by NANOSENSORS
9 October 202220 November 2022
Science & Technology

Figure 2 from Wei Han et al. “Toward Single-Atomic-Layer Lithography on Highly Oriented Pyrolytic Graphite Surfaces Using AFM-Based Electrochemical Etching” Schematic diagram of the AFM-based electrochemical etching apparatus with an RH-controlled environment. The experiments were performed under ambient conditions with a commercial atomic force microscope using NANOSENSORS PtIr5 coated PointProbePlus® PPP-EFM AFM probes. The AFM tip side coating enhances the conductivity of the AFM tip and allows electrical contacts, and the opposite side coating enhances the laser reflex.

Happy #NanotechnologyDay! The date, 10/9, is a nod to the nanometer scale, where objects span only billionths of a meter (10⁻⁹ meters). We celebrate it…

by NANOSENSORS
27 June 202220 November 2022
Science & Technology

Bio-materials typically have an intrinsically low electrical conductivity, and so the availability of a bio-material with extraordinary electrical properties has great potential for new applications…

by NANOSENSORS
3 June 202220 November 2022
Science & Technology

Figure 5 from D. Galanakis et al. “Novel characteristics of soluble fibrin: hypercoagulability and acceleration of blood sedimentation rate mediated by its generation of erythrocyte-linked fibers”: AFM images. Horizontal bars denote 100 nm and 50 nm in panels a and b, respectively. a FR fg adsorbed on a TOMA surface showing a large, upper arrow, and a smaller multimeric cluster, lower arrow, showing the marked variation in the cluster size. The smaller cluster also shows regular surface undulations indicating its multimeric composition. b FR fg adsorbed on modified graphite (MG) surface, showing a field of solitary trinocular monomers and two clusters. Inset: a 4 × magnification from a different area of the same field showing a monomer, right arrow, and a multimeric cluster, left arrow NANOSENSORS SuperSharpSilicon SSS-SEIHR AFM probes were used.

Soluble fibrin (SF) in blood consists of monomers lacking both fibrinopeptides A with a minor population in multimeric clusters. It is a substantial component of…

by NANOSENSORS
16 May 202220 November 2022
News & Events

NANOSENSORS™ PointProbe® Plus AFM probe 3D view - research driven excellence since 1990

NANOSENSORS™ PointProbe® Plus AFM probes will have a tiny TV appearance today on BBC 4 “Secrets of Size: Atoms to Supergalaxies – Going Small” presented…

Scanning Probe Microscopy

Season’s Greetings from NANOSENSORS AFM probes

Male-female communication enhances release of extracellular vesicles leading to high fertility in Drosophila

First day @Arablab

Toward Single-Atomic-Layer Lithography on Highly Oriented Pyrolytic Graphite Surfaces Using AFM-Based Electrochemical Etching

Efficient long-range conduction in cable bacteria through nickel protein wires

Novel characteristics of soluble fibrin: hypercoagulability and acceleration of blood sedimentation rate mediated by its generation of erythrocyte-linked fibers

NANOSENSORS AFM Probes on TV