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Mechanical disassembly of human picobirnavirus like particles indicates that cargo retention is tuned by the RNA-coat protein interaction

Fig. 6 from María J. Rodríguez-Espinosa et al. 2023: Model depicting the RNA cargo retention for each VLP variant. The Δ45-CP VLP structure (A) does not show externalized cargo. The CP VLP structure (B) shows shorter N-terminals compared to the Ht-CP structure (C), which implies less RNA cargo retention compared to the Ht-CP structure. The cartoons in blue, green and pink colors represent coat protein, RNA and N-terminal, respectively. For mechanical fatigue experiments, rectangular AFM cantilevers (NANOSENSORS™, uniqprobe qp-BioAC AFM probes with three different AFM cantilevers per chip) with nominal spring constants of 0.05 and 0.1 N m−1 were used. The AFM cantilevers were calibrated using Sader's method.

The idea of using virus-like particles as nanocarriers for heterologous cargo transport and delivery requires controlling the stability of the container–cargo system.* In particular, the… Read More »Mechanical disassembly of human picobirnavirus like particles indicates that cargo retention is tuned by the RNA-coat protein interaction

Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation

Figure 5 from Catharina Husteden et al. 2023 “Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation”: A–D) Topography images [Cs/Col]4Cs (A), [Cs/Col]4Cs/LPX (B), [Cs/Col]4Cs/LPX/Cs (C), and [Cs/Col]4Cs/LPX/Cs/Col determined by AFM [Scale bar 1 µm] (D). E) Distribution curves of Young's modulus (E0) with a force map of a defined area (see also Figure S4, Supporting Information of the cited article). NANOSENSORSTM uniqprobe triangular qp-BioT AFM probes were used for the quantitative imaging to investigate the surface roughness and topography (in a standard liquid cell of a commercially available atomic force microscope) and force mapping. The uniqprobe qp-BioT AFM probe types offer an alternative to silicon nitride probes, with the advantage of taller AFM tips with smaller opening angles and reduced thermal drift.

The development of smart multifunctional biomaterials with the ability to control the behavior of stem cells on demand has become a powerful strategy in regenerative… Read More »Lipoplex-Functionalized Thin-Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation

Accurate and rapid antibiotic susceptibility testing using a machine learning assisted nanomotion technology platform

Fig. 1 from Alexander Sturm et al. “Accurate and rapid antibiotic susceptibility testing using a machine learning-assisted nanomotion technology platform”: Nanomotion detection and recording platform. a Representation of the components of the nanomotion technology platform. b A representation of the nanomotion measurement setup with the (1) bacteria-loaded cantilever, (2) superluminescent light emitting diode (SLED) = light source, and (3) photodetector. c Schematic illustrating Gram-negative bacteria attached to the cantilever. Prior to attachment, bacteria are dispersed in gelling agarose while the cantilever surface is functionalized using positively charged poly-D-lysine. The gelling agent proved beneficial for an even distribution and stability of the bacterial attachment. d Representative standard 4-h nanomotion recordings with a 2-h medium phase (50% LB medium) followed by a 2-h drug phase with 32 µg/ml CRO for the E. coli reference strains ATCC-25922 (S, susceptible) and BAA-2452 (R, resistant). These recordings form the basis for using nanomotion to conduct AST. This study contains 219 recordings of ATCC-25922 and 225 recordings of BAA-2452 exposed to 32 µg/ml CRO with similar results. Data are available in the source data file. NANOSENSORSTM tipless uniqprobe AFM cantilevers SD-qp-CONT-TL from the NANOSENSORS Special Developments List were used.

Antimicrobial resistance (AMR) has become a significant threat to public health worldwide. * AMR diagnostic strategies such as antibiotic susceptibility testing (AST) help provide clinicians… Read More »Accurate and rapid antibiotic susceptibility testing using a machine learning assisted nanomotion technology platform